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DNA replication and gene expression

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Which RNA directly matches an anticodon to an mRNA codon?

  1. rRNA
  2. mRNA
  3. tRNA
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tRNA

A charged tRNA links codon recognition to delivery of an amino acid.

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Why are RNA primers necessary?

  1. The primer is the enzyme that separates parental strands.
  2. Primers allow one new strand to grow 3-prime to 5-prime.
  3. DNA polymerase requires an existing 3-prime OH to extend.
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DNA polymerase requires an existing 3-prime OH to extend.

A primer supplies an extendable starting end.

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Which feature makes the usual end-replication problem relevant?

  1. Hydrogen bonds between the bases, which break when the strands separate
  2. A circular chromosome with no ends
  3. A linear end with no upstream 3-prime end after primer removal
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A linear end with no upstream 3-prime end after primer removal

This prevents ordinary polymerase from filling that terminal gap.

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Coding DNA is 5-prime ATG CCT 3-prime. Which is the RNA?

  1. 5-prime AUG CCU 3-prime
  2. 5-prime ATG CCT 3-prime
  3. 3-prime UAC GGA 5-prime
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5-prime AUG CCU 3-prime

Replace T with U while retaining coding-strand order and direction.

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A DNA coding strand reads 5' ATG GCA TTC 3'. Which is the mRNA made from this gene?

  1. 5' UAC CGU AAG 3'
  2. 5' AUG GCA UUC 3'
  3. 5' ATG GCA TTC 3'
  4. 3' AUG GCA UUC 5'
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5' AUG GCA UUC 3'

mRNA has the same sequence as the coding strand, with U in place of T, because it pairs with the template strand.

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What is retained after ordinary splicing?

  1. Exons joined in the mature RNA
  2. Only introns, with all exons discarded
  3. Every alternative exon in one universal mature transcript
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Exons joined in the mature RNA

Introns are removed and retained exon regions are linked.

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Which statement correctly relates codon and anticodon?

  1. A tRNA anticodon pairs complementarily with an mRNA codon.
  2. Both are triplets on DNA.
  3. They pair in the same 5-prime to 3-prime direction.
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A tRNA anticodon pairs complementarily with an mRNA codon.

This connects the message to delivery of the corresponding amino acid.

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Which statements about translation are correct? 1: The ribosome moves along mRNA one codon at a time. 2: Each tRNA can carry any amino acid. 3: Peptide bonds form between amino acids. 4: A stop codon is read by a tRNA carrying no amino acid.

  1. 3 and 4 only
  2. 1, 3 and 4 only
  3. 1 and 3 only
  4. 1, 2 and 3 only
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1 and 3 only

The ribosome moves codon by codon and peptide bonds join amino acids. Each tRNA carries one specific amino acid, and stop codons bind release factors.

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Write a short answer, then compare it with a sample and check for missing ideas.

Question 1 of 8

Transcribe and translate a short sequence

3 marks

Template DNA is 3-prime TAC TTT GGC ATT 5-prime. Write the RNA. Given AUG = Met, AAA = Lys, CCG = Pro and UAA = stop, state the peptide. [3]

Keep DNA strand identity and direction explicit.

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