K325 / 2027

Biological molecules

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Which statement correctly pairs a molecule group with its main role?

  1. Protein forms the insulating layer under the skin and is made of glycerol and fatty acids.
  2. Fat provides long-term energy storage and insulation.
  3. Carbohydrate contains nitrogen and is used mainly for the growth and repair of cells.
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Fat provides long-term energy storage and insulation.

Fats contain carbon, hydrogen and oxygen, but differ in structure from carbohydrates.

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Which list correctly gives the smaller units for protein and fat?

  1. Protein: fatty acids and glycerol; fat: glucose and amino acids.
  2. Protein: glucose; fat: amino acids.
  3. Protein: amino acids; fat: glycerol and fatty acids.
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Protein: amino acids; fat: glycerol and fatty acids.

Keep both components when describing the basic units of a fat.

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Benedict's solution stays blue after being added to glucose solution, but the mixture was not heated. What is the best next step?

  1. Conclude that glucose is not a reducing sugar.
  2. Repeat correctly with water-bath heating and appropriate controls.
  3. Add iodine and use its colour to measure the glucose concentration.
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Repeat correctly with water-bath heating and appropriate controls.

Missing heating makes the method incomplete. A known positive control checks that the method and reagent work.

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Invented results for four foods: P: iodine blue-black, Benedict's blue. Q: iodine brown, Benedict's brick-red. R: iodine blue-black, Benedict's green. S: iodine brown, Benedict's blue. Which food contains starch and a small amount of reducing sugar?

  1. S
  2. P
  3. R
  4. Q
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R

Blue-black iodine shows starch, and a green Benedict's result shows a small amount of reducing sugar.

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Which sequence is appropriate for the ethanol emulsion test?

  1. Add biuret reagent, then heat strongly over a flame.
  2. Mix the sample with ethanol, then add the ethanol extract to water.
  3. Shake the sample with water and record any cloudiness as a positive result.
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Mix the sample with ethanol, then add the ethanol extract to water.

A cloudy white emulsion indicates fat. Keep ethanol away from flames and heat sources.

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A student adds biuret solution to egg white, milk, glucose solution and cooking oil. Which pair would turn violet?

  1. Egg white and milk
  2. Glucose solution and milk
  3. Glucose solution and cooking oil
  4. Cooking oil and egg white
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Egg white and milk

Both contain protein, which gives a violet colour with biuret solution.

Check your understanding

Which change explains how an enzyme increases reaction rate?

  1. It lowers activation energy when a suitable substrate forms a complex.
  2. It is used up as it joins the substrate, so more enzyme must keep being made.
  3. It raises the activation energy.
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It lowers activation energy when a suitable substrate forms a complex.

Binding at a complementary active site enables catalysis, and the enzyme remains available for reuse.

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An enzyme is slow at 5 degrees C but becomes active when warmed to 30 degrees C. Which explanation fits?

  1. The cold denatured it, and warming to 30 degrees C let the active site refold into shape.
  2. Cold slowed collisions without permanently changing its active site.
  3. The enzyme died in the cold and came back to life.
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Cold slowed collisions without permanently changing its active site.

The recovered activity supports a reversible effect of cooling rather than permanent denaturation.

Check your understanding

Invented data: amylase takes 120 s to digest starch at 20 degrees C, 40 s at 40 degrees C and 300 s at 60 degrees C. Which statement fits the data?

  1. The enzyme has an optimum of exactly 40 degrees C for all conditions.
  2. The rate is highest at 40 degrees C and falls at 60 degrees C.
  3. The rate at 20 degrees C is three times the rate at 40 degrees C.
  4. The rate is highest at 60 degrees C, because it takes the longest.
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The rate is highest at 40 degrees C and falls at 60 degrees C.

The shortest time means the fastest rate, so 40 degrees C is fastest; 300 s at 60 degrees C is much slower.

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Which method best isolates the effect of pH on amylase activity?

  1. Test each pH at a different temperature, so that the full range of conditions is covered.
  2. Use different pH buffers but keep temperature and enzyme and substrate amounts constant.
  3. Change pH, temperature and the amount of enzyme together.
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Use different pH buffers but keep temperature and enzyme and substrate amounts constant.

A consistent endpoint and repeated measurements then support a fair comparison of pH conditions.

Check your understanding

Why does an amylase experiment include a tube of boiled and cooled amylase with starch?

  1. To keep the temperature of every tube the same.
  2. To give a faster reaction for comparison.
  3. To check that the iodine solution is working and still turns blue-black with starch.
  4. To show that starch disappears only when active enzyme is present.
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To show that starch disappears only when active enzyme is present.

Boiling denatures the enzyme. If starch remains in that tube, the change in the test tubes was caused by active amylase.

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Write a short answer, then compare it with a sample and check for missing ideas.

Question 1 of 10

Compare the molecule groups

3 marks

For carbohydrates, fats and proteins, state their main chemical elements and one main role. Give a second main role for fats. [3]

Use molecule groups rather than food names.

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